mouse anti transforming growth factor β  (Santa Cruz Biotechnology)

Journal: BMC pharmacology & toxicology

Article Title: Bisacurone ameliorates myocardial ischemia/reperfusion injury in rats: regulation of inflammatory and apoptosis pathways via CHOP/GRP78 proteins.

doi: 10.1186/s40360-025-00949-5

Figure Lengend Snippet: Fig. 3 Effect of bisacurone on ischemia-reperfusion injury (IRI)-induced alterations in cardiac TNF-α, IL-1β, and TGF-β mRNA expressions determined by relative quantification of reverse transcriptase-polymerase chain reaction analysis (A), quantitative representation of protein expression of TNF-α (B), IL-1β (C), and TGF-β (D) in rats. Data are expressed as mean [95% CI] (n = 6). *p < 0.05 as compared to the IRI-control group, #p < 0.05 as compared to the sham, $p < 0.05 as compared to one another. Lane 1: Ladder 1000 bp; Lane 2: mRNA expression of sham group; Lane 3: mRNA expression of IRI control group; Lane 4: mRNA expression of IRI + Dil (10); Lane 5: mRNA expression of IRI + Bis (25); Lane 6: mRNA expression of IRI + Bis (50); Lane 7: mRNA expression of IRI + Bis (100) and Lane 8: mRNA expression of per se i.e., Bis (100) treated groups. IRI: Ischemia-reperfusion Injury control rats; Dil (10): diltiazem (10 mg/ kg, p.o.) treated rats; Bis (25): bisacurone (25 µg/kg, p.o.); Bis (50): bisacurone (50 µg/kg, p.o.) and Bis (100): bisacurone (100 µg/kg, p.o.) treated rats. ILs: Interleukins; TNF-α: Tumor necrosis factor-alpha, TGF-β, Transforming growth factor beta

Article Snippet: Briefly, the levels of ANP, Bcl-2, Bax, BNP, cTn-I, caspase-3, heme oxygenase-1 (HO-1), IL-1β, Transforming growth factor-beta (TGF-β), and TNF-α mRNA were analyzed using a qualitative RT-PCR kit (MP Biomedicals India Private Limited, India), as described previously [18].

Techniques: Quantitative Proteomics, Reverse Transcription, Polymerase Chain Reaction, Expressing, Control